Color Combination
Efficient multiplexing approach in Digital PCR
Color combination in Crystal Digital PCR®
In this technical note we will explore how :
- Color combination allows to detect and quantify up to 21 targets in a single reaction with Nio™ Digital PCR.
- Nio™ Analyzer Software unique feature, population editor, allows for single thresholding between positive and negative populations.
- Tips for assay design in color combination: application-specific design strategy, smart assay configuration, and low co-encapsulation rate.
- Color combination approach allows for the development of hyperplexed assays, such as the 18-plex ESR1 (17 mutations) Crystal Digital PCR® Assay.